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Healing skin and Surrounding Nerves
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This study was conducted in order to investigate the relationship between wound healing of skin and their surrounding nerves and how this relationship is reciprocal. Wounds were made on embryos at different stages of development and some embryos studied had no nerves in the area of the wound site. Other studies have suggested that the rate of wound healing is dependent on nerves and that the wound sites become hyperinnervated by sensory nerves during healing. On the other hand, the authors want to confirm that wound healing does not trigger hyperinnervation in the embryo as it does in adults. Fertilized White Leghorn chicken eggs were used and incubated until they reached the embryonic stage 4 (E4) and/or E7. Chick embryos are a good choice because wound healing can occur without scarring or inflammation, which could have an effect on the results. The wing bud was chosen as the site of incision because it is easily accessible for microsurgery and because the nerve pattern in the wing bud is known. The wounds were created by dissecting the wings using an electrolytically sharpened tungsten needle. The surrounding nerves were stained at the incision sites in order to allow the nerve branch points to be seen and counted. Some embryos were made nerveless in order to study any effect this may have on the wound. The embryos chosen to be nerveless were exposed to UV radiation at the E2 stage.
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